Trends In Economic Botany: The Rising Use Of Herbal Supplements :: Botany

Patterns In Economic Botany: The Rising Use Of Herbal Supplements The utilization of home grown solutions for treat medical issues in people is a custom that goes back numerous hundreds of years. A forerunner to present day, Western pharmaceuticals, customary healers utilized herbs to treat a wide scope of diseases and burdens. While many know about their utilization by American Indians, the act of home grown treatment goes back to old Chinese and Egyptian healers. Herbs were utilized in antiquated occasions to treat anything from cerebral pains (with willow bark tea, presently a functioning fixing in headache medicine) to fever and premenstrual condition (with chamomile). During a time of present day pharmaceuticals and their prepared accessibility in Western culture, it is anything but difficult to overlook that around 40% of the present current medications are delivered with synthetic concoctions got from plants (Counter 1998). In a pattern inversion that has the cutting edge clinical network frightened and astounded, the deals of home grown cures in the Untied States has expanded drastically. Generally, Europe has been the biggest market for home grown cures, representing 45% or $7.5 billion in deals for 1997 (Scimone and Scimone 1998). Inside Europe, Germany rules the market with deals of $3.6 billion, trailed by France ($1.8 billion), Italy ($800 million) and the United Kingdom ($300 million) (Scimone and Scimone 1998). Development in the European market was anticipated to be 5-10% in 1998-1999 and 15-20% in 1999-2000 (Scimone and Scimone 1998). The European market has gotten positively settled in the course of recent years, with an unobtrusive development rate until ongoing years that has demonstrated another upward pattern. The United States advertise is a totally unique story. The home grown industry has advanced exponentially in the course of recent years, with critical section into the mass market inside the previous two years (Botanicals International 1998). Deals of home grown enhancements came to $4 billion out of 1998, up from $1.6 billion out of 1994 , an ascent of 250% (American Botanical Council 1998). Deals have been anticipated to increment between 50-100% in 1998-99 and between 20-25% in 2000-01(Scimone and Scimone 1998). What has caused this emotional increment? While an article in the New England Journal of Medicine (1998) accused this inversion to unreasonable methodologies on bafflement with the regularly rushed and indifferent consideration conveyed by traditional doctors, it is likewise seen as the monetary impact of the maturing people born after WW2. As they have gotten more seasoned, this age has become more wellbeing cognizant and progressively disappointed with regular medication in their endeavors to reduce the antagonistic impacts of maturing (Brenneman 1999).

Through the Eyes of a Child free essay sample

Through the Eyes of a Child Why are a few people critical towards others? Regardless of whether we judge on race, sex, or something as basic as age, making a decision about causes anguish as well as can leave passionate scars individuals never recoup from. Bigotry is one of the most well-known of critical structures despite everything seen today in our general public. Albeit today we don't discover bigotry as noticeable as, thinking back to the 1920s and 1930s. When perusing the story â€Å"The Angel of the Candy Counter† composed by Maya Angelou, we can see the harm of bigotry from a child’s view, and how she managed the involvement with request to effectively permit us to comprehend the negative intensity of prejudice. Because of Angelou fusing techniques, for example, ethos, poignancy, stunning words, and even dreams to bring out exceptional feelings she can interface with her crowd. Unmistakably Angelou composes as though she is addressing a wide scope of crowd. She promptly opens recounting to an account of a kid feeling just as she is being rebuffed with a toothache: â€Å"The Angel of the Candy Counter had discovered me finally, and was demanding horrendous atonement for all the taken Milky Ways, Mounds, Mr. Goodbars and Hersheys with Almonds† (146). Angelou later states, â€Å"It appears to be frightfully uncalled for to have a toothache and a cerebral pain and need to hold up under simultaneously the overwhelming weight of Blackness† (147). With these two explanations we can finish up she is concentrating on youngsters and ethnic minorities, for both can set up a shared opinion with what they are perusing. Then again, she accentuates the bigotry being depicted by white individuals, and with this we can close she is attempting to show how demonstrations of prejudice influence others wanting to open the eyes of her white crowd to the issue. In spite of the fact that, as a result of the wide scope of crowd that will peruse her story, particularly her white crowd, Angelou may need to recollect whether she isn't cautious with her wording, she may estrange or outrage her perusers in the incorrect manner which may overcome her goal. The immediate reason for â€Å"The Angel of the Candy Counter† is to permit Angelou, inside a story setting, to pull on our heart strings, unsettle up our otherwise calm disposition as it were, or even displeasure us so as to give a knowledge of how disparaging and putting down bigotry is. Angelou gives us how changing reality into dream empowers her to manage the dismissal of the white dental specialist. She shows fulfillment in fantasizing how her grandma faced the dental specialists as opposed to permitting him the option to betray them. Angelou’s dream depicts how her grandma â€Å"walked in that room as though she possessed it† (148), and continued to tell the dental specialist how he would leave town and never practice dentistry again. This would be equity given to what Angelou calls â€Å"the insidious white man† (149). At a certain point or another we ourselves may have discovered the utilization of getting away into dream so as to manage our torments. The way Angelou utilizes dream empowers us to interface with her and makes a shared opinion. Angelou’s motivation behind ethos is to additionally build up a shared opinion among herself and her perusers, for instance, â€Å"Annie, you know I don’t treat nigra, hued people† (148). Utilizing this explanatory system, Angelou gives her crowd the feeling of estrangement and embarrassment she felt, her involvement in the dental specialist, and his demonstrations of prejudice. This is significant on account of the expansive scope of crowd she is addressing. She depends essentially on the expectation we at once or another have encountered some type of estrangement or embarrassment in our lives. This structures a shared belief relationship, builds up respectability of her insight into realizing her crowds own encounters, and helps in making a common trust between her crowd and herself. The utilization of tenderness permits Angelou to effectively summon an enthusiastic reaction in her crowd, regardless of whether she makes outrage, feel sorry for, the sentiment of shamefulness, or down-right disfavor she needs to attract us closer to the sentiments of her own inadequacy as a youthful dark kid. Angelou notes, â€Å"If one was kicking the bucket, it must be done in style if the perishing occurred in whitefolks’ part of town† (147). Angelou utilizes this talk, passing on to us how a dark individual must present themselves within the sight of a white individual. Another way Angelou depicts her sentiments of inadequacy, among white individuals, is standing out she subtleties how charming the white people’s part of town is, for example, â€Å"The rock street was smoother, the stones littler and the tree limbs hung down around the way and almost secured us† (147). While perusing these announcements Angelou’s grown-up crowd for instance, more so then others, may feel rankled by the acknowledgment of a little youngster being compelled to feel immaterial and contemptible. She can utilize the image of a youngster, realizing grown-ups will in general shield kids from sentiments of despondency. In doing as such, she has caused an association with her grown-up crowd by blending them to up sincerely in a security mode. Angelou can utilize this association as a guide in achieving her purpose behind composition. In utilizing stunning language all through her story, Angelou allows her crowd to sympathize with her agony in an increasingly significant way for instance, â€Å"Annie, my arrangement is I’d preferably stick my turn in a dog’s mouth over a nigger’s† (148). Utilizing these solid words empowers Angelou to show how the most excruciating of toothaches can't come close to the weight of being dark. These words are intentionally planned to incite outrage and a feeling of mistrust in order to by and by interface with Angelou on a more profound level. Angelou needs her crowd to recognize the agony she feels and all the more so recognize how awful prejudice is. Now, we may now ask ourselves, for what reason are a few people critical towards others? This is an inquiry with no simple or positive answer. We can just hypothesize that possibly it is some type of feeling of inadequacy which causes them to feel they have to put down others to improve their own sentiments of prevalence. In spite of the fact that, our general public anyway has figured out how to decrease the passes up our development of acknowledgment towards one another, there are the individuals who despite everything have far to go.

Can Marijuana Help Your Bipolar Disorder

Can Marijuana Help Your Bipolar Disorder Bipolar Disorder Treatment Print Can Marijuana Help Your Bipolar Disorder? By Marcia Purse Marcia Purse is a mental health writer and bipolar disorder advocate who brings strong research skills and personal experiences to her writing. Learn about our editorial policy Marcia Purse Medically reviewed by Medically reviewed by Steven Gans, MD on January 09, 2020 Steven Gans, MD is board-certified in psychiatry and is an active supervisor, teacher, and mentor at Massachusetts General Hospital. Learn about our Medical Review Board Steven Gans, MD Updated on January 15, 2020 Bipolar Disorder Overview Symptoms & Diagnosis Causes Treatment Living With In Children Your Rights Deux / The Image Bank / Getty Images Award-winning mental health journalist and author,  John McManamy, wrote a thoughtful blog about the implications of medical marijuana as a treatment for bipolar. Its reasonable to think that the risks outweigh any possible benefits, but the topic is certainly worth discussing. Since both bipolar depression and mania can have psychotic features, there is some evidence that even medical marijuana use might have negative effects in people with bipolar disorder.?? Studies Show Links to Worse Outcomes Medical research shows that cannabis use in people with psychosis is associated with an earlier age of their first psychotic episode. Its also associated with manic symptoms and problems thinking.?? In one study, patients who quit using marijuana or reduced its use following their first psychotic episode had the greatest improvement in symptoms at the one-year mark, compared both to continuing cannabis users and people who had never used cannabis.??  Long-term cannabis use may have a negative effect on long-term clinical outcomes for those with bipolar spectrum disorders, as well. A 2015 study found lower bipolar disorder remission rates for current regular cannabis users (those who used it three times a week or more often) and those who regularly smoke tobacco when compared to people who dont use either substance. That study, which lasted two years, concluded that regular marijuana users who also have bipolar didnt do as well long-term as people who didnt use the drug.?? Another study looked at the short-term effects of cannabis use in people with bipolar disorder  and concluded that the drug was associated with both manic and depressive symptoms. However, that study couldnt find evidence that people with bipolar were using cannabis to self-medicate on a regular basis.?? Now, none of these studies prove that cannabis is actually causing these problems in people with bipolarâ€"they just show an association between marijuana use and problems. But you should factor this information into your thinking when deciding whether or not to use cannabis. Substance Abuse Risk With Marijuana All drugs have risks and side effects, and cannabis is no exception. Substance abuse can be quite prevalent among those with bipolar disorder. People have used alcohol and drugs to try to control their systems in great numbers  and may reduce their likelihood of successful treatment of their bipolar as a result. By using marijuana to self-medicate for bipolar disorder, you run the risk of gaining a second diagnosis in addition to your bipolar diagnosis: substance abuse (Substance use disorder).

Battle of Ligny During the Napoleonic Wars

The Battle of Ligny was fought on June 16, 1815, during the Napoleonic Wars (1803-1815). Heres a summary of the event. Battle of Ligney Background Having crowned himself Emperor of the French in 1804, Napoleon Bonaparte embarked on a decade of campaigning which saw him win victories at places such as Austerlitz, Wagram, and Borodino. Finally defeated and forced to abdicate in April 1814, he accepted exile on Elba under the terms of the Treaty of Fontainebleau. In the wake of Napoleons defeat, the European powers convened the Congress of Vienna to outline the postwar world. Unhappy in exile, Napoleon escaped and landed in France on March 1, 1815. Marching to Paris, he built an army as he traveled with soldiers flocking to his banner. Declared an outlaw by the Congress of Vienna, Napoleon worked to consolidate power as Britain, Prussia, Austria, and Russia formed the Seventh Coalition to prevent his return. Armies and Commanders Prussians Field Marshal Gebhard von Blà ¼cher84,000 men French Napoleon Bonaparte68,000 men Napoleons Plan Assessing the strategic situation, Napoleon concluded that a swift victory was required before the Seventh Coalition could fully mobilize its forces against him. To achieve this, he sought to destroy the Duke of Wellingtons coalition army south of Brussels before turning east to defeat Field Marshal Gebhard von Blà ¼chers approaching Prussian army. Moving north, Napoleon divided his Armee du Nord (Army of the North) in three giving command of the left-wing to Marshal Michel Ney, the right-wing to Marshal Emmanuel de Grouchy, while retaining personal command of a reserve force. Understanding that if Wellington and Blà ¼cher united they would have the power to crush him, he crossed the border at Charleroi on June 15 with the intention of defeating the two coalition armies in detail. That same day, Wellington began directing his forces to move towards Quatre Bras while Blà ¼cher concentrated at Sombreffe. Determining the Prussians to pose a more immediate threat, Napoleon directed Ney to seize Quatre Bras while he moved with the reserves to reinforce Grouchy. With both coalition armies defeated, the road to Brussels would be open. The next day, Ney spent the morning forming his men while Napoleon joined Grouchy at Fleurus. Making his headquarters at the windmill of Brye, Blà ¼cher deployed Lieutenant-General Graf von Zietens I Corps to defend a line running through the villages of Wagnelà ©e, Saint-Amand, and Ligny. This formation was supported by Major General George Ludwig von Pirchs II Corps to the rear. Extending east from I Corps left was Lieutenant General Johann von Thielemanns III Corps which covered Sombreffe and the armys line of retreat. As the French approached on the morning on June 16, Blà ¼cher directed II and III Corps to send troops to reinforce Zietens lines. Napoleon Attacks To dislodge the Prussians, Napoleon intended to send forward General Dominique Vandammes III Corps and General Étienne Gà ©rards IV Corps against the villages while Grouchy was to advance on Sombreffe. Hearing artillery fire coming from Quatre Bras, Napoleon commenced his attack around 2:30 PM. Striking Saint-Amand-la-Haye, Vandammes men carried the village in heavy fighting. Their hold proved brief as a determined counterattack by Major General Carl von Steinmetz reclaimed it for the Prussians. Fighting continued to swirl around Saint-Amand-Haye through the afternoon with Vandamme again taking possession. As the loss of the village threatened his right flank, Blà ¼cher directed part of II Corps to attempt to envelop Saint-Amand-le-Haye. Moving forward, Pirchs men were blocked by Vandamme in front of Wagnelà ©e. Arriving from Brye, Blà ¼cher took personal control of the situation and directed a strong effort against Saint-Amand-le-Haye. Striking the battered French, this assa ult secured the village. Fighting Rages As fighting raged to the west, Gà ©rards men hit Ligny at 3:00 PM. Enduring heavy Prussian artillery fire, the French penetrated the town but were ultimately driven back. A subsequent assault culminated in bitter house-to-house fighting which resulted in the Prussians maintaining their hold on Ligny. Around 5:00 PM, Blà ¼cher directed Pirch to deploy the bulk of II Corps south of Brye. At the same time, a degree of confusion struck the French high command as Vandamme reported seeing a large enemy force approaching Fleurus. This actually was Marshal Comte dErlons I Corps marching in from Quatre Bras as requested by Napoleon. Unaware of Napoleons orders, Ney recalled dErlon before he reached Ligny and I Corps played no role in the fighting. The confusion caused by this created a break which allowed Blà ¼cher to order II Corps into action. Moving against the French left, Pirchs corps was stopped by Vandamme and General Guillaume Duhesmes Young Guard Division. The Prussians Break Around 7:00 PM, Blà ¼cher learned that Wellington was heavily engaged at Quatre Bras and would be unable to send aid. Left on this own, the Prussian commander sought to end the fighting with a strong attack against the French left. Assuming personal oversight, he reinforced Ligny before massing his reserves and launching an assault against Saint-Amand. Though some ground was gained, French counterattacks forced the Prussians to begin retreating. Reinforced by General Georges Moutons VI Corps, Napoleon began assembling a massive strike against the enemy center. Opening a bombardment with sixty guns, he ordered troops forward around 7:45 PM. Overwhelming the tired Prussians, the attack broke through Blà ¼chers center. To halt the French, Blà ¼cher directed his cavalry forward. Leading a charge, he was incapacitated after having his horse shot. The Prussian cavalry was soon halted by their French counterparts. Aftermath Assuming command, Lieutenant-General August von Gneisenau, Blà ¼chers chief of staff, ordered a retreat north to Tilly after the French broke through at Ligny around 8:30 PM. Conducting a controlled retreat, the Prussians were not pursued by the exhausted French. Their situation improved quickly as the newly-arrived IV Corps deployed as a strong rearguard at Wavre which allowed a rapidly-recovering Blà ¼cher to reassemble his army. In the fighting at the Battle of Ligny, the Prussians sustained around 16,000 casualties while French losses numbered around 11,500. Though a tactical victory for Napoleon, the battle failed to mortally wound Blà ¼chers army or drive it to a location from which it could no longer support Wellington. Forced to fall back from Quatre Bras, Wellington assumed a defensive position where on June 18 he engaged Napoleon at the Battle of Waterloo. In heavy fighting, he won a decisive victory with the aid of the Blà ¼chers Prussians which arrived in the afternoo n.

Discussion Research On Parent Involvement Essay - 805 Words

Prior Research on Parent Involvement in Education Before turning to our qualitative study of parent involvement in urban char - ter schools, the following sections outline the prior research on the benefits of parent involvement, the barriers to involvement that exist, and the potential of the charter school context to reduce these barriers. Benefits of Parent Involvement Decades of research point to the numerous benefits of parent involvement in education for not only students but also for the parents involved, the school, and the wider community (Barnard, 2004; Epstein, 2001; Fan Chen, 2001; Henderson Mapp, 2002; Jeynes, 2003, 2007; Lee Bowen, 2006). De - spite the challenges in establishing a causal link between parent involvement and student achievement, studies utilizing large databases have shown positive and significant effects of parent involvement on both academic and behavioral outcomes (Fan Chen, 2001; Jeynes, 2003, 2007). For example, research has found that parent involvement is related to a host of student achievement indi - cators, including better grades, attendance, attitudes, expectations, homework completion, and state test results (Astone McLanahan, 1991; Cancio, West, Young, 2004; Dearing, McCartney, Weiss, Kreider, Simpkins, 2004; Gut - man Midgley, 2000; Izzo, Weissberg, Kasprow, Fendrich, 1999; Senechal LeFevre, 2002; Sheldon, 2003). Additional academic outcomes such as lower dropout rates (Rumberger, 1995), fewerShow MoreRelatedInfluence Of Parenting Styles And Practices Globally1302 Words   |  6 Pagesstyles and practices globally. The attitude and response of parents to various parenting practices is based on the knowledge or information they are exposed to or available to them. This study intends to examine the influence of education on parent’s involvement in raising their children especially outside of school. 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Sanitation of Rooms and Equipments (Microbiology) Free Essays

There are Four Methods that conducted on the laboratories in order to detect the presence of microorganisms. There are Rodac Method, Swab Method, Rinse Method, and lastly Open Dish Method and it will be discussed in detail below. 2. We will write a custom essay sample on Sanitation of Rooms and Equipments (Microbiology) or any similar topic only for you Order Now 1. 1 Rodac Method The purpose of this Standard Operating Procedure is to describe a program that will adequately measure the efficacy of disinfection of Rooms and equipment in each laboratory, RODAC plates can detect the presence or absence of live microorganisms (Longree and Armbruster 1996). This Method is used to monitor the contamination level of personnel gowns and Personal Protective Equipment (PPE) before or during manufacturing production. The advantages of the RODAC method are that it may be prepared and stored for weeks prior to use (Harrigan 1986). Additional advantages of the RODAC method include relatively low cost, consistent and precise recovery, effective use by personnel without extensive training, and the elimination of laboratory manipulation after sampling (Marriott and Gravani 2006). On the other hand, the disadvantages of this method are the spreading of the colonies and applicable to only limited to low levels of surface contaminants. 2. 1. 2 Swab Method The Swab method is among the most Reproducible Methods used to determine the population of microorganisms present on equipment or food products (Marriott and Gravani 2006). It may be used to assess the amount of contamination from the air, water, surfaces, facilities and food products. By using this technique the equipment surfaces, facilities and food products which to be analyzed are swabbed. The swab are diluted in a dilutant such as peptone water or phosphate buffer, according to the anticipated amount of contamination and subsequently applied to a growth medium containing agar in a sterile, covered plate (David, Richard and R. 2004). There are many advantages to the cotton swab method. These include the ease with which any health care provider can procure the necessary items: a CTA or culturette transport medium (Longree and Armbruster 1996). In addition, the method requires little expertise, with minimal training time required, and very little time required to actually perform the procedure. On the other hand, Disadvantages of the swab method are that sampling and technique can affect the results and that the method requires manipulation to culture the sample. Swabs are designed for hard-to-reach places, and can fit easily into equipment recesses, nooks, and crevices (Tamime 2008). After collection of the sample, it is recommended that a standard membrane filtration of the rinse solution be conducted. 2. 1. 3 Rinse Method The Rinse Method use elution of contamination by rinsing to permit a microbial assay of the resultant suspension (Forsythe 2008). A sterile fluid is manually or mechanically agitated over an entire surface. The rinse fluid then diluted and subsequently plated, this method are more precise compared to the swab method, because a larger surface area can be tested (David, Richard and R. 2004). While the disadvantages is that it requires time and labor to prepare solutions and media, dilute samples, pour plate samples, and count colony-forming units on the plates. 2. 1. 4 Open Dish Method The principle behind this method is that the bacteria carrying particles are allowed to settle onto the medium for a given period of time and incubated at the required temperature. A count of colonies formed shows the number of settled bacteria containing particles (David, Richard and R. 2004). In this method petri dishes containing an agar medium of known surface area are selected so that the agar surface is dry without any moisture. Choice of the medium depends upon the kind of microorganisms to be enumerated. For an overall count of pathogenic, commensal and saprophytic bacteria in air blood agar can be used (Longree and Armbruster 1996). For detecting a particular pathogen which may be present in only small numbers, an appropriate selective medium may be used. Malt extract agar can be used for molds. The plates are labeled appropriately about the place and time of sampling, duration of exposure etc. Then the plates are uncovered in the selected position for the required period of time. The optimal duration of exposure should give a significant and readily countable number of well isolated colonies, for example about 30-100 colonies (McLandsborough 2003). Usually it depends on the dustiness of air being sampled. In occupied rooms and hospital wards the time would generally be between 10 to 60 ‘minutes (McLandsborough 2003). During sampling it is better to keep the plates about I meter above the ground. Immediately after exposure for the given period of time, the plates are closed with the lids. Then the plates are incubated for 24 hours at 37Â °C for aerobic bacteria and for 3 days at 22Â °C for saprophytic bacteria (McLandsborough 2003). 2. 2 Group of microbes that often exist in the room and equipment The normal tendency of a microbial cell when it comes in contact with a solid surface is to attach itself to the surface in an effort to compete ef? ciently with other microbial cells for space and nutrient supply and to resist any unfavorable environ-mental conditions (Adams and Moss 2000). Under suitable conditions, almost all microbial cells can attach to solid surfaces, which are achieved through their ability to produce extracellular polysaccharides. As the cells multiply, they form micro colonies, giving rise to a bio? lm on the surface containing microbial cells, extracellular polysaccharide glycocalyx, and entrapped debris. In some situations, instead of forming a bio? lm, the cells may attach to contact surfaces and other cells by thin, thread like exopolysaccharide materials, also called ? mbriae (Lappin-Scott and J. 1995). Attachment of microorganisms on solid surfaces has several implications on the overall microbiological quality of food. Microbial attachment to and bio? lm formation on solid surfaces provide some protection of the cells against physical removal of the cells by washing and cleaning. These cells seem to have greater resistance to sanitizers and heat. Thus, spoilage and pathogenic microorganisms attached to food surfaces, such as carcasses, ? sh, meat, and cut fruits and vegetables, cannot be easily removed by washing, and later they can multiply and reduce the safety and stability of the foods (Hui 2003). Similarly, microbial cells attached to a culture broth. These places, in turn, can be a constant source of undesirable microorganisms to foods handled in the environment. The concept and importance of microbial attachment and bio? lm formation in solid food, equipment, and food environments are now being recognized (Loken 1995). Limited studies have shown that under suitable conditions, many of the microorganisms important in food can form a bio? lm. Several species and strains of Pseudomonas were found to attach to stainless steel surfaces, some within 30 min at 25oC to 2 hour at 4oC (Stanga 2009). Listeria monocytogenes was found to attach to stainless steel, glass, and rubber surfaces within 20 min of contact. Attachment of several pathogenic and spoilage bacteria has also been demonstrated on meat and carcasses of poultry, beef, pork, and lamb (Stanga 2009). The microorganisms found to attach to meat surfaces include Lis. monocytogenes, Micrococcus spp. , Staphylococcus spp. , Clostridium spp. , Bacillusspp. , Lactobacillus spp. , Brochothrix thermosphacta, Salmonella spp. , Escherichiacoli, Serratia spp. , and Pseudomonas spp (Tamime 2008). It is apparent from the limited data that microbial attachment to solid food and food contact surfaces is quite wide and needs to be considered in controlling the microbiological quality of food. Several possible mechanisms by which microbial cells attach and form a bio? lm on solid surfaces have been suggested. One suggestion is that the attachment occurs in two stages. In the ? rst stage, which is reversible, a cell is held to the surface by weak forces (Cramer 2006). In the second stage, a cell produces complex polysaccharide molecules to attach its outer surface to the surface of a food or equipment, and the process is irreversible. A three-step process that includes adsorption, consolidation, and colonization has been suggested by others (Cramer 2006). In the reversible adsorption stage, which can occur in 20 min, the cells attach loosely to the surface. During the consolidation stage, the microorganisms produce threadlike exopolysaccharides ? mbriae and ? rmly attach the cells to the surface. At this stage, the cells cannot be removed by rinsing (Marriott and Gravani 2006). In the colonization stage, which is also irreversible, the complex polysaccharides may bind to metal ions on equipment surfaces and the cells may metabolize products that can damage the surfaces. The level of attachment of microorganisms to food-processing equipment surfaces is found to be directly related to contact time. As the contact time is prolonged, more cells attach to the surface, the size of the microcolony increases, and attachment between cells increases (Loken 1995). Fimbriae formation by the cells occurs faster at optimum temperature and pH of growth. Limited studies also showed that when microorganisms such as Pseudomonas fragi and Lis. monocytogenes are grown together, they form a more complex bio? lm than when either is grown separately (Stanga 2009). Bibliography Adams, M. R. , and M. O. Moss. Food Microbiology. Winnipeg: Royal Society Of chemistry, 2000. Cramer, Michael M. Food Plant Sanitation: Design, Maintenance, and Good Manufacturing Practices. New York: CRC Press, 2006. David, McSwane, Linton Richard, and Rue Nancy R. Essentials of Food Safety and Sanitation. New York: Prentice Hall, 2004. Entis, Phyllis. Food Safety: Old Habits and New Perspectives. ASM Press, 2007. Forsythe, Stephen J. The Microbiology of Safe Food. Wiley-Blackwell, 2008. Harrigan, Wilkie F. Laboratory Methods in Food Microbiology. Chicago: Academic Press, 1986. Hui, Yiu H. Food plant sanitation. Marcel Dekker Press, 2003. Lappin-Scott, Hilary M. and J. William Costerton. Microbial Biofilms . Cambridge University Press, 1995. Loken, Joan K. The HACCP Food Safety Manual. New York: Wiley Publisher, 1995. Longree, Karla, and Gertrude Armbruster. Quantity Food Sanitation. London: Wiley, 1996. Marriott, Norman G. , and Robert B. Gravani. Principles of Food Sa nitation. Springer Press, 2006. McLandsborough, Lynne. Food Microbiology Laboratory. New York: CRC Press, 2003. Stanga, Mario. Sanitation: Cleaning and Disinfection in the Food Industry. Wiley-VCH Verlag GmbH, 2009. Tamime, Adnan. CLEANING-IN-PLACE: Dairy, Food and Beverage Operations. Wiley-Blackwell Publisher, 2008. Anita How to cite Sanitation of Rooms and Equipments (Microbiology), Papers

Love Marriage vs Arrange Marriage Essay Example

Love Marriage vs Arrange Marriage Essay Whenever we talk of Indian wedding, we try to associate it with arranged marriages. Due to the social structure, the concept of arranged marriage is prevalent in the Indian wedding scenario, since ages. On the other hand, love marriages were considered as a taboo among many Indian people, who do not have a modern outlook of life. For them, two people should tie the wedding knot only with the consent of their parents and the blessings of their relatives. Nonetheless, love marriages are prevalent in almost all the societies of India, given the fact that they are still considered inferior to the weddings arranged by many parents in the country. People supporting the concept of love marriage strongly believe that it is very important to know the partner before marrying him/her. On the other hand, people who believe in solemnizing the wedding with the permission of parents and relatives think that arranged marriages are long lasting. In this article, we have discussed love marriage vis-a-vis arranged marriage. ProsCons: It is said that compromise is a factor that decides whether the marriage would work out or not. In case of love marriage, people might expect more from their partner, largely because they have fallen in love before marriage. This leads to lesser compromises, as the person expects more from his/her partner. On the other hand, compromise and adjustments form the foundation of arranged marriage, largely because the married couple does not have any preconceived notions or expectations from one another. The compromise factor might work wonders in case of most of the arranged marriages, while in love marriages, that might prove to be yet another cause for altercation. Due to this factor, people consider arranged marriage as long lasting and better than love marriage. In case of arranged marriage, the married couple could resort to their parents or acquaintance at the time of financial crises or other problems. In addition, if the marriage proves to be a failure, they have a number of people around them to put seek support or to put the blame on. Their parents would come forward to solve the problems between the couple, if they have married with the elders consent. This is the reason why arranged marriages are considered secure for the people in India. We will write a custom essay sample on Love Marriage vs Arrange Marriage specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Love Marriage vs Arrange Marriage specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Love Marriage vs Arrange Marriage specifically for you FOR ONLY $16.38 $13.9/page Hire Writer On the other hand, the couples who have solemnized love marriage would have to tackle all their crises on their own, because they might have been separated from their family. Resentment drives the parents and the relatives to remain dormant in case the married couple wants any financial or moral support a common sight seen in love marriages in India. Due to this factor, many people do not want to marry without their parents consent, because they would be ultimately cut off from the family ties.

50 Topics for Impromptu Student Speeches

50 Topics for Impromptu Student Speeches For the many people who break out in a sweat at the very idea of speaking in front of an audience, the prospect of speaking on an unknown topic with little to no preparation is likely terrifying. But you dont have to be afraid of impromptu speeches. As it turns out, the secret even to off-the-cuff speeches is preparation. Impromptu Speech Tips Decide on your topicCome up with three supportive statements related to your topicPrepare a strong conclusion Use this list of impromptu speech topics to practice making a quick speech outline in your head. For each of the topics below, just think of three main points youd like to make. For example, if your speech topic is Your least favorite chores, you could quickly come up with three statements: I dont know anybody who likes to fold laundry, so the first task on my list of unhappy chores is folding laundry.Taking out the trash is another chore that most people dread, and Im no different.The worst chore in the entire household has to be cleaning the toilet. If you go into your speech with these statements in your head, you can spend the rest of your time thinking up supporting statements as you speak. When youve identified your three main points, think of a great finishing statement. If you end with a great closer, youll really impress your audience. Start Practicing With This List My three favorite animals.What you would find in my closet. Make something up.What youd find under my bed.The best letter of the alphabet.Why your mom/dad is special.A day that stands out.The best surprise ever.I lost it!If I had a million dollars to give away.If cats/dogs ruled the world.A trip to remember.My favorite day of the year.If I could only eat three foods forever.If I could design a school.Why books are important.Three surprising facts about me.How to impress your parents.How to plan a party.A job Id love to have.A day in my life.If I could have dinner with anyone.If I could travel through time.My favorite book.An important lesson Ive learned.What Ive learned from cartoons.The smartest cartoon character.Three things Id change if I ruled the world.Why sports are important.The worst chores at home.Why I deserve an allowance.If I were in charge of school lunches.If I had invented school.The best theme park rides.Whom do you admire most?What is your favorite animal?How to achi eve your dreams. Why you need a baby brother.How to annoy an older sister.How to save money.Three things that scare me.Great things about snow days.Things you can make out of snow.How to spend a rainy day.How to walk a dog.Great things about the ocean.Things Ill never eat.How to be a slacker.Why I like my town.The best parts of a parade.Interesting things you see in the sky.Things to remember when youre camping.An experience with a bully.

5 Online Courses on Advanced PowerPoint Training

5 Online Courses on Advanced PowerPoint Training 5 Online Courses on Advanced PowerPoint Training Advanced PowerPoint training is important for college students. However, finding the right course is equally essential because you actually need to learn some useful skills. Below you can find the list of 5 online courses that will help you to learn the ropes of the key advanced PowerPoint features: Microsoft PowerPoint Course by Virtual Training Company With this training, students can learn to create presentations, edit presentations and collaborate on them no matter where they are. Students can make their ideas really shine when they integrate various fonts and themes for their presentations, incorporate animations, and even in bed videos. PowerPoint provides students with multiple templates for different reports, portfolios and educational presentations that they have to make. Eye-Catching Presentations Using PowerPoint by Udemy With this course, students can look over different templates from other users and they can create new presentations from anywhere even if they dont have an Internet connection by way of using their phone, tablet, or computer. Students can work collectively at the same time on a single PowerPoint slide. PowerPoint 2016 (Office 365) by Learn IT Anytime This particular training is great for students who are more advanced in their PowerPoint familiarity. Students who are tasked with preparing a handful of slides which they will contribute to a larger group presentation can all work together on the same presentation document. They can collaborate in real-time, make edits, keep track of their edits and revisions, and communicate with other users while they are doing it. All of the changes which are made are sorted by date and by the person who made the different changes so any reviews versions of the same presentation can be viewed by every user. Beginner to Pro in PowerPoint by Udemy Students who need specialized training for a certain class, say, in finance or business, can learn all about the different charts and graph features available to presenting data. If one student is using PowerPoint for their contribution to the group project, it can be opened by other students, edited, and saved to the presentation. In addition to this, students can present their final work without a huge set up. PowerPoint is supported by almost any device in the classroom. Microsoft PowerPoint Course by Alison With this training, students will learn to use shortcuts, templates, and all of the inbuilt features of the program. Students and teachers can insert images and videos into the presentation which are relevant to the demonstration of their knowledge and subject, and all of this information can be published within a website. This extends far beyond the classroom and allows administrators and teachers alike to create presentations and then post them to a classroom or academic institution webpage that is created through the same Google applications. This information might be something applicable to the student body, to a department, or to a classroom individually. In the end, PowerPoint functions as a presentation application which allows students and teachers to show off the work that theyve completed in a very visually appealing manner. This way students, teachers, and administrators alike can create presentations and edit them as they prefer. They can edit existing presentations with friends, peers, or coworkers, and share an existing presentation with one another in an effortless fashion. Users can import PowerPoint files of any format and convert them to a presentation. All presentations can be downloaded as a presentation format using a PDF, PowerPoint, JPEG, or text file. Overall, you can always contact professional PowerPoint presentation writing services provided by our company.

Enforceability of international and local mediation outcome Assignment

Enforceability of international and local mediation outcome - Assignment Example A normal mediation session takes three hours to complete and is attended by both the parties. Mediation is fair, efficient and does not require lengthy litigation and investigation. II. ROLE OF THE MEDIATOR The role of the mediator differs in four different phases of mediation.1 He generally is a contract facilitator. The different roles of the mediator are mentioned below; A. The pre-mediation phase This is considered as the most important part of the mediation process. In this phase, the mediator can create a strong base for mediation process. He has to see if the case is suitable for mediation or not. Some cases cannot be resolved through mediation like criminal matters2 thus he has to see whether the mediation should proceed or not. The mediator also has to check the flexibility of both the parties as the end result of the mediation will be ‘a contract’3 and thus it has to be assessed whether both the sides have the capacity to enter into a contract or not. The last thing that has to be checked is the representation of both the parties, the mediator has to check that the representatives are representing according to the law and their authority over the party has to be checked, so that it should be in the mediator’s knowledge that what kind of agreement the representatives can sign. B. ... e mediator engages both the parties in a detailed discussion in which both of them try to understand each other’s point of view and perspective; the third duty, which is being performed by the mediator in this session, is to influence the parties to settle the dispute, to make them reach an agreement and to resolve the dispute; and the last task, that a mediator performs during this session, is to engage both the parties in a thorough discussion regarding the issues which they have agreed upon. During this session the parties discuss past and present agendas, whereas, discussing future issues is optional, even though, the discussion of the future issues is essential for this session as it enables the parties to understand each other’s interests and goals. After this session the mediator and both the parties will be aware of each other’s point of views, goals and interests. Each party will now be able to frame a proposal that will satisfy its interests. In additio n to that, it will also be able to put forward an offer that will be acceptable by the other party. The mediator will now help the parties to check the accessibility and practicability of their offers. C. The separate session ( the private session) In this stage the mediator tries to detect and put forth the issues that the parties did not discuss in the last session, to enable the parties to reach realistic options that satisfy the needs and interests of both the parties and to guide the parties to negotiate on these options in the upcoming session. The role played by mediator in this phase includes the following; firstly, the mediator has to ensure both the parties that the private session is strictly confidential and therefore they can discuss the issues and agendas which they did not discuss in the

HR services Essay Example | Topics and Well Written Essays - 1000 words

HR services - Essay Example While there are a great variety of users of human resource services (HR services) within an organization, this essay will identify three key users, identify two key needs for each user, and explain how they can be prioritized One use of the human resource department is through the hiring and firing of employees. In these regards, all aspects of the organization rely on the human resource department to ensure that employees are screened properly and when there are significant disciplinary problems that they be released. In some regards, the human resource department can confronted with conflicting needs in this category; in these regards, it’s necessary for the department to attend to an urgent need, but in most instances handle these issues in the order they occurred. Another user of human resources is upper level management, through employee record keeping; within this service the primary needs are for the use of employee records, while keeping the records confidential from m embers of the organization that are not privy to them. In these regards, it’s necessary for HR services to keep stringent record keeping, but also to remain highly cognizant of which organizational members are privy to the specific recorded content. Other users of HR services within an organization are entry-level employees. These employees use HR services for both training programs and compensation and benefit management. In some regards, there may be a conflict of interest here, as the same individuals responsible for training the department are responsible for determining compensation bonuses. In these regards, it’s necessary to have an outside department, generally management, conduct reports that will factor into employee progress reports and ultimately bonuses. 2. Identify at least 3 methods of communication appropriate to employees at different levels ? Among the different methods of communication appropriate for employees at different levels, this essay will co nsider three main types (‘Workplace Communication’). The first type of communication is verbal communication. Verbal communication represents the most direct and perhaps effective form of communication. The drawback of verbal communication is that it does not leave a record of the conversation that took place, nor is it always possible for upper level management to verbally address all the concerns of entry level, or low-level employees. In these regards, another level of communication that is effective is written communication. Written communication functions to produce a clear and understandable message in a context that allows for the individuals implementing this communication process to ensure there is a record of their communication. In these regards, written communication is important in matters wherein the individual receiving the message is of high importance. It’s also effective when the message is complex and it would be useful to be able to review the message. The final type of communication that is considered is visual communication. Generally visually communication is implemented through presentations from upper level to lower level employees, but it can also be implemented in presentations that speak to upper level organizational members, as a means of informing them of status updates, or proposing potential changes. Ultimately, visual communication is most effective for speaking to larger audiences, or in terms of explicating difficult to comprehend material through chart forms; finally it can demonstrate business prototypes or models highly effectively that couldn’t otherwise be articulated through verbal or written communication methods. 3. Describes the key components of effective service delivery including: ? Service delivery has taken on a variety of contextual meanings, making clear and precise definition of the various aspects of the

Hplc Analysis Of Aloe Vera Tablets Biology Essay

Hplc Analysis Of Aloe Vera Tablets Biology Essay The project work was aimed to achieve the quantitative determination of aloin and aloe emodin in the form of tablets by employing HPLC. The method used was reverse phase high performance liquid chromatography. Calibration curve method was used for the quantification of aloin and aloe emodin. The mobile phase was the mixture of acetonitrile and deionised water in the ratio of 60:40 respectively. The mobile phase was pumped at 1.5 ml/minute and the analyte was quantified at the wavelength of 220 and 296nm. The column used for separation was kromasil 5C18. Reverse phase Isocratic run of standard aloin and standard aloe emodin was done and the peaks obtained from their analysis were used to compare the test sample peaks. Aloe vera colax tablets manufactured by Aloe pura laboratories were used as the test sample tablets which were extracted with water, methanol, acetonitrile, methanol-water and acetonitrile-water. After extraction they were subjected for isocratic run in HPLC instrument a nd the data obtained were compared with that of the standard. CHAPTER 1 INTRODUCTION 1.1 Introduction to Aloe Vera Aloes is the dried juice of the leaves of Aloe barbadensis Miller, known as Curacao aloes, or of Aloe perryi Baker known as Socotrine aloes, or of Aloe ferox Miller and hybrids of the species of Aloe africana Miller and Aloe spicata Baker, known as Cape aloes belonging to the family Liliaceae. [2,3] The synonym of aloes is Aalwee, Aalwyn, Kumari, Star cactus, Aroe, Acibar, Babosa, etc. [1] Aloes is indigeneous to eastern and southern Africa and grown in Cape colony, Zanzibar and islands of Socotra. It is also cultivated in Caribbean islands, Europe and many parts of India, including North West Himalayan region. [2] All the varieties of aloe are the major sources of anthraquinone glycosides. The principal active composition of aloe is aloin, which is a mixture of glucosides, among which barbaloin is the chief constituent. It is chemically aloe-emodin anthrone C-10 glucoside and is water-soluble. [2] Barbaloin is a C- glycoside and it is not hydrolysed by heating with dilute acids or alkalies. Ferric chloride decomposes barbaloin by oxidative hydrolysis into aloe-emodin-anthrone, little aloe-emodin and glucose. [2] Along with barbaloin, aloes also contains isobarbaloin, b-barbaloin, aloe-emodin and resins. The drug also contains aloetic acid, homonataloin, aloesone, chrysophanic acid, chrysamminic acid, galactouronic acid, choline, choline salicylate, saponins, mucopolysaccharides, glucosamines, hexuronic acid, coniferyl alcohol, etc. [2] The amount of barbaloin in different commercial varieties varies to a large extent. Curacao aloes contain about 22 percent of barbaloin. Indian variety, generally Aloe vera contain very less quantity (3.5 to 4 percent). Curacao aloes contains two and half times quantity of aloe-emodin , compared to Cape-aloe-emodin. [2] The resin of aloe principally contains Aloesin. It is a type of C- glucosyl chromome. Aloesin is also responsible for purgative action of aloes. [2] Fig. 1 Fig. 2 Aloin [5] Aloe emodin [6] 1.2 Uses of Aloe Vera: Aloes is used as purgative. Its effect is mainly on colon. It has a stronger purgative action in the series of all crude drugs with anthracene glycosidal content. To counter effect the gripping action, it is given along with carminatives. [2] It facilitates the healing of any kind of skin wound, burn, or scald even speeding recovery time after surgery. [4] It is applied topically in acne, sunburn, frostbite (it appears to prevent decreased blood flow), shingles, screening out x-ray radiation, psoriasis, preventing scarring, rosacea, warts, wrinkles from aging, and eczema. [2, 4] It also seems to help prevent opportunistic infections in cases of HIV and AIDS due to its immune system stimulant properties. [4] It appears to be of help in cancer patients (including lung cancer) by cativating white blood cells and promoting growth of non- cancerous cells. [4] Aloe also appears to work on heartburn, arthritis, and rheumatism pain and asthma. [2, 4] It also lowers the blood sugar levels in diabetics. [2, 4] Other situations in which it appears to work when taken internally inclue congestion, internal worms, indigestion, stomach ulcers, colitis, hemorrhoids, liver problems such as cirrhosis and hepatitis, kidney infections, urinary tract infections, prostate problems, and as a general detoxifier. [2, 4] CHAPTER 2 HPLC 2.1 HPLC: Introduction and Instrumentation The technique of high performance liquid chromatography is so called because of its improved performance when compared to classical column chromatography. It is also called as high-pressure liquid chromatography since pressure is used when compared to classical column chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressure of up to 400 atmospheres. For the separation, identification and quantification of compounds, this method is frequently used in biochemistry and analytical chemistry. [11, 12] The development of HPLC from classical column chromatography can be attributed to the development of smaller particle sizes. Smaller particle size is important since they offer more surface area over the conventional larger sizes. [7] 1960s 40 to 60m 1970s 10 to 20m 1980s 5 to 10m 1990s 1 to 3m A porous particle of 5m offers a surface area of 100-860 sq.metres/gram with an average of 400 sq.metres/gram. These offer very high plate counts upto 100,000/metre. Table 1: Comparison of classical column chromatography with HPLC [7] Parameter Classical column chromatography HPLC Stationary phase particle size Large 60-200m Small 3-20m Column size Length x int. diameter Large 0.5-5m x 0.5-5cm i.d. Small 5-50cm x 1-10mm i.d. Column material Glass Mostly metal Column packing pressure Slurry packed at low pressure often gravity Slurry packed at high pressure >5000 psi Operating pressure Low ( High (500 3000 psi) Flow rates Low to very low Medium to high (Often >3ml/min) Sample load Low to medium (g/mg) Low to very low (mg) Parameter Classical column chromatography HPLC Cost Low High Detector flow cell volume Large 300 to 1000ml Low 2 to 10ml Column efficiency i.e. Resolving power (Low) Theoretical plates per meter (High) often >100,000 Plates per meter Types of stationary phases available Limited range Wide range Scale of operation Preparative scale Analytical and preparative scale 2.2 Types of HPLC techniques [7, 9, 10, 11, 12] Based on Modes of Chromatography There are two modes viz. Normal phase mode and Reverse phase mode. These modes are based on the polarity of stationary phase and mobile phase. Before explaining the modes, it is important to know the interactions, which occur between solute, stationary and mobile phase. Polar Polar interaction or affinity is more Nonpolar Nonpolar interaction or affinity is more Polar Nonpolar interaction or affinity is less Normal phase mode: In normal phase mode, the stationary phase (eg. Silica gel) is polar in nature and the mobile phase is non-polar. In this technique, non-polar compounds travel faster and are eluted first. This is because of less affinity between solute and stationary phase. Polar compounds are retained for longer time in the column because of more affinity towards stationary phase and take more time to be eluted from the column. This is not advantageous in pharmaceutical applications since most of the drug molecules are polar in nature and takes longer time to be eluted and detected. Hence this technique is not widely used in pharmacy. Reverse phase mode: In reverse phase technique, a non-polar stationary phase is used. The mobile phase is polar in nature. Hence polar components get eluted first and non-polar compounds are retained for a longer time. Since most of the drugs and pharmaceuticals are polar in nature, they are not retained for a longer time and eluted faster, which is advantageous. Different columns used are ODS (Octadecyl silane) or C18, C8, C4, etc. Common reverse phase solvents are methanol, acetonitrile, tetrahydrofuran and water. Based on principle of separation Adsorption chromatography Ion exchange chromatography Ion pair chromatography Size exclusion or Gel permeation chromatography Affinity chromatography Chiral phase chromatography Each of the above technique is described in brief as follows: Adsorption chromatography: The principle of separation is adsorption. Separation of components takes place because of the difference in affinity of compounds towards stationary phase. This principle is seen in normal phase as well as reverse phase mode, where adsorption takes place. Ion exchange chromatography: The principle of separation is ion exchange, which is reversible exchange of functional groups. In ion exchange chromatography, an ion exchange resin is used to separate a mixture of similar charged ions. For cations, a cation exchange resin is used. For anions, an anion exchange resin is used. Ion pair chromatography: In ion pair chromatography, a reverse phase column is converted temporarily into ion exchange column by using ion pairing agents like pentane or hexane or heptane or octane sulphonic acid sodium salt, trtramethyl or tetraethyl ammonium hydroxide, etc. Size exclusion or gel permeation chromatography: In this type of chromatography, a mixture of components with different molecular sizes is separated by using gels. The gel used acts as molecular sieve and hence a mixture of substances with different molecular sizes is separated. Soft gels like agarose , dextran or polyacrylamide are used. Semi rigid gels like polystyrene, alkyl dextran in non-aqueous medium are also used. The mechanism of separation is by steric and diffusion effects. Affinity chromatography: Affinity chromatography uses the affinity of the sample with specific stationary phases. This technique is mostly used in the field of Biotechnology, Microbiology, Biochemistry, etc. Chiral phase chromatography: Separation of optical isomers can be done by using chiral stationary phases. Different principles operate for different types of stationary phases and for different samples. The stationary phases used for this type of chromatography are mostly chemically bonded silica gel. Based on elution technique 1. Isocratic separation: In this technique, the same mobile phase combination is used throughout the process of separation. The same polarity or elution strength is maintained throughout the process. In this technique, the peak width increases with retention time linearly according to the equation for N, the number of theoretical plates. Gradient separation: In this technique, a mobile phase combination of lower polarity or elution strength is used followed by gradually increasing the polarity or elution strength. One example is a gradient starting at 10% acetonitrile and ending at 90% acetonitrile after 25 minutes. The two components of the mobile phase are termed as A and B. Where A is the weak solvent and B is the strong solvent. Weak solvent allows the solute to elute slowly while strong solvent rapidly elutes the solutes from the column. A is usually water where as B is an organic solvent which is miscible with water such as acetonitrile, methanol, THF or isopropanol. Based on scale of operation 1. Analytical HPLC: Where only analysis of the samples are done. Recovery of the samples for reusing is normally not done, since the sample used is low. Eg. mg quantities. 2. Preparative HPLC: Where the individual fractions of pure compounds can be collected using fraction collector. The collected samples are reused eg. Separation of few grams of mixtures by HPLC. Based on type on analysis 1. Qualitative analysis: Which is used to identify the compound, detect the presence of impurities, to find out the number of components, etc. This is done by using retention time values. 2. Quantitative analysis: Which is done to determine the quantity of the individual or several components in a mixture. This is done by comparing the peak area of the standard and sample. 2.3 Principle of separation in HPLC: [7, 9] The principle of separation in normal phase and reverse phase mode is adsorption. When a mixture of components is introduced in to a HPLC column, they travel according to their relative affinities towards the stationary phase. The component, which has more affinity towards the adsorbant, travels slower. The component, which has less affinity towards the stationary phase, travels faster. Since no two components have the same affinity towards the stationary phase, the components are separated. 2.4 Instrumental Requirements [7, 9, 10, 12] Pumps solvent delivery system Mixing unit, gradient controller and solvent degassing Injector Manual or auto injectors Guard columns Detectors Recorders and integrators Fig. 3 The schematic diagram of HPLC [13] 1. Pump Solvent delivery system The solvents or mobile phases used must be passed through the column at high pressure at about 1000 to 3000 psi. This is because as the particle size of stationary phase is few m (5 10m), the resistance to the flow of solvent is high. Hence such high pressure is recommended. There are different types of pumps available. They are mechanical pumps and pneumatic pumps. A mechanical pump operates with constant flow rate and uses a sapphire piston. This type of pump is used in analytical scale. Pneumatic pumps operate with constant pressure and use highly compressed gas. The solvents used must be of high purity, preferably HPLC grade and filtered through 0.45m filter. Check valves: These are present to control the flow rate of solvent and back pressure. Pulse dampners: These are used to dampen the pulses observed from the wavy baseline caused by the pumps. 2. Mixing unit, gradient controller and solvent degassing Mixing unit is used to mix solvents in different proportions and pass through the column. There are two types of mixing units. They are low pressure mixing chamber, which uses helium for degassing solvents. High pressure mixing chamber does not require helium for degassing solvents. Mixing of solvents is done either with a static mixer, which is packed with beads, or dynamic mixer, which uses magnetic stirrer and operates under high pressure. Gradient controller In an isocratic separation, mobile phase is prepared by using pure solvent or mixture of solvents, i.e. solvent of same eluting power or polarity is used. But in gradient elution technique, the polarity of the solvent is gradually increased and hence the solvent composition has to be changed. Hence a gradient controller is used when two or more solvent pumps are used for such separations. Solvent degassing Several gases are soluble in organic solvents. When solvents are pumped under high pressure, gas bubbles are formed which will interfere with the separation process, steady baseline and the shape of the peak. Hence degassing of the solvent is important. This can be done by using any one of the following technique. Vacuum filtration which can remove all air bubbles. But it is not always reliable and complete. Helium purging i.e. by passing helium through the solvent. This is very effective but helium is expensive. Ultrasonication by using ultrasonicator, which converts ultra high frequency to mechanical vibrations. This causes the removal of air bubbles. 3. Injector Manual or auto injectors Several devices are available either for manual or auto injection of the sample. Different devices are: Septum injectors for injecting the sample through a rubber septum. This is not common, since the septum has to withstand high pressure. Stop flow (on line) in which the flow of mobile phase is stopped for a while and the sample is injected through a valve device. Rheodyne injector (Loop valve type) It is the most popular injector. This has a fixed volume loop like 20ml or 50ml or more. Injector has two modes, i.e. load position when the sample is loaded in the loop and inject mode, when the sample is injected. 4. Guard column Guard column has very small quantity of adsorbent and improves the life of the analytical column. It also acts as a prefilter to remove particulate matter, if any, and other material. Guard column has the same material as that of analytical column. Guard column does not contribute to any separation. 5. Analytical columns Analytical column is the most important part of HPLC technique, which decides the efficiency of separation. There are several stationary phases available depending upon the technique or mode of separation used. Column material: The columns are made up of stainless steel, glass, polyethylene and PEEK (Poly ether ether ketone). Most widely used are stainless steel, which can withstand high pressure. Latest ones are PEEK columns. Column length: Varies from 5cm to 30cm Column diameter: Ranges from 2mm to 50mm Particle size: From 1m to 20m Particle nature: Spherical, uniform sized, porous materials are used. Surface area: 1 gram of stationary phase provides surface area ranging from 100 860 sq.m with an average of 400 sq.m. Functional group: the functional group present in stationary phase depends on the type of chromatographic separation. In normal phase mode it contains the silanol groups (hydroxy group). In reverse phase mode it contains the following groups: C18 Octa Decyl Silane (ODS) column C8 Octyl column C4 Butyl column CN Nitrile column NH2 Amino column For other modes of chromatography, ion exchange columns, gel columns, chiral columns, affinity chromatographic columns, etc. are available. 6. Detectors [7,9,10] Detectors used depend upon the property of the compounds to be separated. Different detectors available are UV detector: This detector is based upon the light absorption characteristics of the sample. Two types of this detector are available. One is the fixed wavelength detector, which operates at 254nm where most drug compounds absorb. The other is the variable wavelength detector, which can be operated from 190nm to 600nm. Refractive index detector: This is a non-specific or universal detector. This is not much used for analytical applications because of low sensitivity and specificity. Flourimetric detector: This detector is based on the fluorescent radiation emitted by some class of compounds. The exitation wavelength and emission wavelength can be selected for each compound. This detector has more specificity and sensitivity. The disadvantage is that some compounds are not fluorescent. Conductivity detector: Based upon electrical conductivity, the response is recorded. This detector is used when the sample has conducting ions like anions and cations. Amperometric detector: This detector is based on the reduction or oxidation of the compounds when a potential is applied. The diffusion current recorded is proportional to the concentration of the compound eluted. This is applicable when compounds have functional groups, which can be either oxidised or reduced. This is a highly sensitive detector. Photodiode array detector (PDA detector): This is a recent one, which is similar to UV detector, which operates from 190 600nm. Radiations of all wavelengths fall on the detector simultaneously. The resulting spectrum is a 3-D or three-dimensional plot of Response Vs Time Vs Wavelength. The advantage is that the wavelength need not be selected, but the detector detects the responses of all the compounds. 7. Recorders and integrators Recorders: They are used to record the responses obtained from detectors after amplification, if necessary. They record the baseline and all the peaks obtained, with respect to time. Retention time for all the peaks can be found out from such recordings, but the area of individual peaks cannot be known. Integrators: Integrators are improved version of recorders with some data processing capabilities. They can record the individual peaks with retention time, height, and width of peaks, peak area, percentage of area, etc. Integrators provide more information on peaks than recorders. Now a days computers and printers are used for recording and processing the obtained data and for controlling several operations. 2.5 Parameters used in HPLC [7, 9, 10] Retention time (Rt): Retention time is the difference in the time between the point of injection and appearance of peak maxima. Retention time is the time required for 50% of a component to be eluted from a column. Retention time is measured in minutes or seconds. Retention time is also proportional to the distance moved on a chart paper, which can be measured in cm or mm. Retention volume (Vr): Retention volume is the volume of mobile phase required to elute 50% of the component from the column. It is the product of retention time and flow rate. Retention volume = Retention time x flow rate Separation factor (S): Separation factor is the ratio of partition co-efficient of the two components to be separated. It can be expressed and determined by using the following equation: S = Kb/ Ka = Ka/ Kb = (tb t0)/ (ta t0) Where, t0 = Retention time of unretained substance Kb, Ka= Partition coefficients of b and a tb, ta = Retention time of substance b and a S = depends on liquid phase, column temperature If there is more difference in partition coefficient between two compounds, the peaks are far apart and the separation factor is more. If the partition coefficients of two compounds are similar, then the peaks are closer and the separation factor is less. Resolution: Resolution is a measure of the extent of separation of two components and the baseline separation achieved. It can be determined by using the following formula: Rs = 2 (Rt1 Rt2)/ (W1 +W2) Theoretical plate (Plate theory): A theoretical plate is an imaginary or hypothetical unit of a column where distribution of solute between stationary phase and mobile phase has attained equilibrium. A theoretical plate can also be called as a functional unit of the column. HETP Height Equivalent to a Theoritical Plate [18, 7] A theoretical plate can be of any height, which decides the efficiency of separation. If HETP is less, the column is more efficient. If HETP is more, the column is less efficient. HETP can be calculated by using the following formula: HETP = length of the column/ number of theoretical plates HETP is given by Van Deemter equation HETP = A + (B/u ) + Cu Where, A = Eddy diffusion term or multiple path diffusion which arises due to packing of the column. This is unaffected by mobile phase velocity or flow rate. This can be minimised by uniformity in packing. B = Longitudinal diffusion term or molecular diffusion which depends on flow rate. C = Effect of mass transfer which depends on flow rate. u = Flow rate or velocity of the mobile phase. A column is efficient only when HETP is minimum. Hence an ideal flow rate corresponding to the minimum value of HETP is used. Efficiency (No. of theoretical plates): The number of theoretical plates expresses efficiency of a column. It can be determined by using the formula: n = 16 Rt ²/w ² Where, n = no. of theoretical plates Rt = retention time w = peak width at base Rt and w are measured in common units (mm or cm or minutes or seconds) and are proportional to the distances marked on chart paper. If the number of theoretical plates is high, the column is said to be highly efficient. If the number of theoretical plates is low, the column is said to be less efficient. For gas chromatographic columns, a value of 600/ metre is sufficient. But in HPLC, high values like 40,000 to 70,000/ metre are recommended. Asymmetry factor: A chromatographic peak should be symmetrical about its centre and said to follow Gaussian distribution. In such cases, the peak will be like an isosceles triangle. But in practice, due to some factors, the peak is not symmetrical and shows tailing or fronting. Fronting is due to saturation of stationary phase and can be avoided by using less quantity of sample. Tailing is due to more active adsorption sites and can be eliminated by support pre-treatment, more polar mobile phased increasing the amount of liquid phase. Asymmetry factor (0.95 to 1.05) can be calculated by using the formula: AF = b/a (b and a calculated at 5% or 10% of the peak height) 2.6 Applications of HPLC HPLC is being more widely used in several fields. Apart from its use in Pharmaceutical field, it is used in Chemical and Petrochemical industry, Environmental applications, Forensic applications, Biochemical separations, Biotechnology, Food analysis, etc. In fact there is no field where HPLC is not being used. It is a versatile and sensitive technique, which can be used in several ways. Some of them are listed below: Qualitative analysis: It is nothing but identification of compound. This is done by comparing the retention time of the sample as well as the standard. Under identical conditions, the retention time of the standard and the sample are same. If there is a deviation, then they are not the same compound. Checking the purity of the compound: By comparing the chromatogram of the standard and that of the sample, the purity of the compound can be inferred. If additional peaks are obtained, impurities are present and hence the compound is not pure. From the percentage area of the peaks obtained, the percentage purity can also be known. Presence of impurities: This can be seen by the presence of additional peaks when compared with a reference standard or reference material. The percentage of impurities may also be calculated from peak areas. Quantitative analysis: The quantity of a component can be determined by several methods like a. Direct comparison method By injecting a sample and standard separately and comparing their peak areas, the quantity of the sample can be determined. Area of the peak = peak height x width of peak at the half height A1/ A2 = a (W1/ W2) Where, A1 and A2 are peak area of sample and standard W1 and W2 are weight or concentration of sample and standard a is the response factor b. Calibration curve method: In calibration curve method, series of standards are used to determine their peak areas. A calibration curve of peak area Vs concentration of the drug is plotted. From the peak area of the unknown sample, by intrapolation, the concentration of the sample can be determined. This method has the advantage that errors, if any, are minimised. Internal standard method: In this method, a compound with similar retention characteristics is used. A known concentration of the internal standard is added to the sample solution whose concentration is not known. The chromatogram is recorded and their peak areas are determined. By using formula, the concentration of unknown solution is determined. Multicomponent analysis or Determination of mixture of drugs: Similar to the quantification of a single drug, multicomponent analysis can be done easily. The quantity of each component is determined by using any one of the above methods. Marketed formulations, which contain several drugs, can be determined quantitatively for each component. Isolation and identification of drugs or metabolites in urine, plasma, serum, etc. can be carried out. Isolation and identification of mixture of components of natural or synthetic origin. Biopharmaceutical and Pharmacokinetic studies. Stability studies. Purification of some compounds of natural or synthetic origin on preparative scale. 2.7 Limitations: [7, 10] The limitations of HPLC are that drugs have to be extracted from their formulations prior to analysis and large amounts of organic solvent waste are generated which are expensive to dispose off. CHAPTER 3 Experimental Selection 3.1 Aim of Project: The aim of this project was to carry out the quantitative determination of the active pharmaceutical ingredient aloin and aloe-emodin in the given Aloe Vera Colax tablets, manufactured by Aloe Pura laboratories and to compare the results with the given standard aloin and aloe-emodin. The technique used for analysis was reverse phase High Performance Liquid Chromatography method. The analysis was performed using standard calibration curve generated at 220 and 296nm wavelength. 3.2 Chromatographic equipment and conditions: All the chromatographic equipments and conditions, which were used to perform HPLC in a laboratory environment under simulated GLP compliance conditions, are listed below. 3.2.1 HPLC system 5 (used for isocratic elution): This system is manufactured by Agilent technologies 1200 series, whose model number is G1310A and the serial number is DE 62956545 3.2.2 Software used: The software used was Microsoft windows XP, Pentium D whose product number is G 2175 BA, revision code is B. 03. 01 and its registration number is CL1CE8DB0F 3.2.3 Column used: The column used was Kromasil 5C18 whose test number is 9203- 10344 3.2.4 Pipette used: The pipette used was Volac ultra (made in U.K.), S. No. 29186, Model: R680/ F, 0-1000 mL and Volac ultra (made in U.K.), S.No. 29185, Model: R680/ F, 500-5000 mL. 3.2.5 Analytical Balance: Mettler balance AC 88 was used to weigh the sample drug whose Biom

Management Case study Essay

Case Study 1. How do information technologies contribute to the business success of the companies depicted in the case? Provide an example from each company explaining how the technology implemented let to improved performance. Information technologies are imperative to businesses and organizations that want to be successful. In this case, there were three companies that demonstrated how information technologies helped their business. The first company was eCourier. eCourier delivers packages around London and wanted to â€Å"keep real-time tabs on customer satisfaction†(Textbook, pg 5). eCourier used SeeWhy software to help give them customer data faster. This software allowed eCourier to see when client’s accounts were going dormant or if there were changes in the bookings. With this new data, eCourier saved money by not having to add extra staff to monitor who was happy with service and who was not. The second company was Cablecom. Cablecom was also interested customer satisfaction. They used statistical software to look through customer data and filter out trouble. They also found that using survey research in combination with statistical software gave a more accurate look at customer satisfaction or dissatisfaction. These two information technologies allowed Cablecom to see which customers were unhappy and helped them retain those customers. Finally, the third company was a law firm called Bryan Cave. Bryan Cave needed an alternative to the hourly fee normally charged by law firms. Clients wanted â€Å"fixed pricing and pricing that was adjusted during a project† (Textbook, pg 6). The law firm had spreadsheets to help breakdown the collection of fees and how much profit was being made but these spreadsheets were difficult and confusing. The company used business intelligent tools to help â€Å"lawyers track budgets in real time so that they could quickly make adjustments†(Textbook, pg. 6). The use of this technology led to improved profitability for the law firm and also improvement in hours worked by fee earners in the law firm. 2. In the case of law firm Bryan Cave discussed above, the use of BI technology to improve the availability, access, and presentation of existing  information allowed them to provide tailored and innovative services to their customers. What other professions could benefit from a similar use of these technologies, and how? Develop two different possibilities. Many professions can use business intelligence tools just like in the case of Bryan Cave law firm. The banking industry can use BI tools to stay competitive. With competition nature and increasing due regulatory legislation surrounding financial institutions, it is imperative that financial institutions monitor customer’s behaviors related to consumption of banking products in an effort to retain the customers. Business intelligence tools are being developed and marketed to banks to help monitor, analyze accounts, and determine customer profitability. This type of business intelligence tool would help the banks market to current customers based on their propensity to purchase products based on transaction behavior and other products the customers currently utilize. Additionally, a business intelligience tool that performs an analysis of the current fee structure charged for existing products in relation to the competition in the marketplace would help the bank determine if their pricing structure is competitive and in line with the competition. This technology would also help financial institutions identify which customers are profitable and whether or not fees should be increased or reduced. Currently, Chemical Bank has implemented a business intelligience tool that commerical lenders will utilize by cross rreferencing their loan portfolio and any prospect against to determine the profitability of the customer in relation to the company’s return on equity. The business intelligience tool will help the commerical lenders determined if the loan rate is in line given certain parameters such as the market, financial statements, and the customer’s business plan. Additionally, the tool will help determine if packaging a suite of products would benefit the customer if more products and services of the bank were utilized. Finally, Chemical Bank is investigating an innovative business intelligence tool called data cube is being considered for implementation. The data cubes would be developed from different reports for regional sales managers allowing them to manipulate the data to be more useful strategic planning and determining staffing solutions. BS&A software  company could benefit from business intelligence tools. BS& A provides tax software and assessing software to many Michigan townships and cities. Each platform communicates with any of the other BS& A platforms. From personal experience it is a wonderful program. To help retain current clients BS& A could use survey research to see what needs to be enhanced for each client or what needs to be simplified for each client. This would allow the company to then tailor its software to fit each customer’s needs. At the township I work, we needed a way to enter credit card payments into our tax program from BS & A. The only payments accepted in the software were checks and cash. By filling out the survey and sending it back via internet I was immediately contacted and the program writers made the necessary changes that allowed me to enter when a client of the township paid with a credit card. I was very satisfied with the results and BS & A retained my township as one of their clients. Case Study 3: Cablecom developed a prediction model to better identify those customers at risk of switching to other company in the near future. In addition to those noted in the case, what other actions could be taken if that information were available? Give some examples of these. Would you consider letting some customers leave anyway? Why? Cablecom used two softwares, SPSS’s statistical software and SPSS’s Dimensions survey research software, to study customer dissatisfaction. SPSS’s statistical software tells: 1. How many times problem occurred 2. How long does it took to fix the problem Drawbacks of this software: This may or may not tell what the problem was. SPSS’s Dimensions survey research software tells: 1. After how many months of service customers gets dissatisfied- 9 months 2. When customers switch most- between 12-14 months Drawback: why to wait till 7-9 months to find the problem? Actions to prevent switching: Find the problem, fix the problem: Send monthly satisfaction surveys to find the problem areas. If people are not responding very well then add some incentives to it like lucky prizes from the drawings of responders. Be PROACTIVE 1. Keep customers engaged by prroviding some money saving tips to customers with their monthly bill. Some 2. examples of tips that may improve customer retention are: family plans over individual plans that would allow for a cost savings; Explain the benefit of texting if it’s cheaper than making phone calls; explain the benefits of bundled plans over individual plans for phone, cable and TV; tips to increase the life of the products that Cablecom provides. Newsletters about new products and services, explaining how these are useful to customers and how they are better than competition. Brining a value added benefit to customers on a monthly or quarterly basis will create an affinity for the services a company provides. I will not consider letting some customers leave anyway, unless those are financially unsustainable on long-term basis. There are different reasons for this: 1. 2. 3. 4. 5. If customer is there then job is available. It takes lots of advertising resources to get one customer. One satisfied customer brings more customers by word of mouth. One unsatisfied customer talks bad about company more loudly than a satisfied  customer talks good about company. Your competitor is getting your business. Question 9. In what major ways have the roles of information systems applications in business expanded during the last 40 years? What is one major change you think will happen in the next 10 years? The roles of information systems applications in business evolved in following order during the last 40 years: ï‚ · ï‚ · Technical changes or Data Processing: Until 1960 information systems was used for transactions, processing, record keeping, accounting. Managerial changes (1960s to 80’s): 1. Management reporting (1960’s): MIS helped in making managerial decisions from pre defined managerial 2. reports. Decision Support System (1970’s): Introduced to help management make decisions from the available raw data using unique inputs and style. ï‚ · Corporate Activities (1980’s to 2000’s): 1. Strategic end user support (1980’s): This includes end user computing, executive information for critical 2. 3. decisions, use of artificial intelligence for strategy building, expert systems for providing knowledge to end user and strategic information on how to place products and services in competitive world. Enterprise Resource Planning (2000’s): It includes every aspect of business ranging from manufacturing, supply, sales, HR, Inventory management, marketing etc. This also uses business Intelligence for better insight into competitor activities. Enterprise beyond vendors and customers or global Networking (1990’s): Introduction of e-commerce, ebusiness, development of internet, intranet and extranet. In future 10 years, speed and integrity of Information system will be crucial. This includes solving the problem of ethics in business, storage of personal information and data, missing or incorrect information. Some examples of the challenges include insider trading in stock market, corruption in news corporations, credit card and insurance companies. P.S.: I am having a hard time in fixing bullets and numbering on this page editor. It inserts numbers automatically when not needed and does not accepts bullets when needed. So proper formatting is a challenge unless I type on this page. Any suggestions or ideas to fix this problem are welcome. Gubir, I had the same problem with formatting so related to indenting. Insead of typing this editor, I have been typing in a Microsoft word document, to copy and paste the text over. It allows me to do a better spell and grammar check as well. ~Lisa Question 1 How can information technology support a company’s business processes and decision making and give it a competitive advantage? Give examples to illustrate your answer. As stated in the textbook, â€Å"information technology can help support all types of business by improving efficiency and effectiveness of businesses processes and decision making† (Textbook, p.4). Incorporating information technology early in the decision making process and working collaboratively with the different business units will provide opportunities to strengthen a company’s competitive position by creating innovative solutions to deliver in the marketplace. Additionally, it’s imperative to involve information technologist specialists or strategists  (depending on the title designated by companies) at the very early stages of decision making processes. Understanding the role of IT in the managerial decision making process will help the manager make better decisions that provide a competitive edge. For example, a manager of retail store deciding which product line to add to a store may analyze computer generated reports to support his or her decision as the goal is to remain profitable and have an edge over the local competitor. The manager would demonstrate poor decision making and leadership by reviewing reports after purchasing the product line. Carrying a product line that customers will not purchase does not make good business sense. Further, managers involved in a company’s strategic planning process should seek the knowledge and support of information technology specialists as well to determine the role information technology will play in developing and implementing the company short term and long term strategic initiatives. Information technology systems can be an essential ingredient in business processes such as product development, customer support processes, and ecommerce transactions (Textbook, pp. 8-9). For example, as a Retail Sales Manager for Chemical Bank, information technology representatives work collaboratively with me and other teams we develop new initiatives and projects to support the company’s strategic plans. Most recently, the company embarked on a mission to increase the services and products of the current customers at penetration rate of 70%. A manager is sought the support IT as I created training of my staff to achieve the goal and also to develop the reporting of data to monitor progress toward the goal. Without a robust product line and customer service centers to meet the needs of the customers, financial institutions are not profitable or competitive. Information technology helps financial institutions stay in tune with the customer’s needs, wants and pulse of the industry. Question 2. How does the use of the Internet, intranets, and extranets by companies today support their business processes activities? The Internet, intranets, extranets, and the technologies that support the  applications have changed the business processes, the productivity and communication of employees, and helped companies participate in a global marketplace. As a starting place, it’s imperative to evaluate how the Internet, intranets and extranets have revolutionized the work environment and productive of the employees driving businesses. Employees now ability to work virtually and collaborating with different work groups or teams from across the company via e-mail, videoconferencing, e-discussion groups without geographic barriers. By utilizing intranets (networks within the company or enterprise), employees can support business applications from anywhere they have access or connectivity to an intranet. For example, employees at the financial institution I am employed, Chemical Bank, can access loan documents needed to close a loan for customer instantly from a cue on their desktop instead of waiting for an inter-office mail delivery from the loan department. All 142 branches have equal access in real time for loan documentation. Obviously, the speedier process of delivering loan document supports the bank’s profitability by getting the loans on the book quicker as well as providing better customer service because there is less wait time for the customer. Additionally, employee work more efficiently and the exchange of ideas flows better with the use of the intranet for internal projects and the extranet for interfacing and exchange of ideas with customers. The extranet (the network or enterprise between the business and the customers/vendors) provides for the business and the employees the infrastructure to support business applications vital to many companies and the ability to collaborate among workgroups. (Textbook, p.12) Further, the Internet has benefits and supports businesses today by establishing a medium for the exchange of not only ideas but also goods and services. E-Commerce is defined by the book as â€Å"the buying, selling, marketing, and servicing of products, services, and information over a variety of computer networks†. (Textbook, p. 13) Businesses utilize the Internet in every step of the process involved in selling a service or good. Businesses advertise and market the goods and services on the Internet to promote the brand loyalty. Additionally, the ability to purchase goods on line and make payments increases the sales and profitability of companies. For example, Kohl’s department stores now have kiosks placed in their stores. Customers who cannot find a size of a particular item or merchandise that is no longer in  stock at one location utilizes the kiosk for service. The kiosk is an internet based selling system that allows customers to purchase items using their Kohl’s charge with all applicable discounts and the item will be sent to the customer’s home within a very short period of time. In years prior, customers would try to locate a sales representative who would contact another store to locate the item wanted which could take up to twenty minutes. Then, the option is given to the customer to pick up the item at another store that has it or have the merchandise delivered to a local store requiring another trip by the customer. The process was cumbersome and didn’t provide the customer with an exception customer service experience. The kiosk on the other hand provides instant service and creates customer loyalty. Finally, the market research and data that the Internet provides can support a company all the way from product development and innovation to the level of service being delivered by the employees on the front line. All of which is very important to businesses in making managerial decisions and deciding business processes. For example, financial institutions such as Chemical Bank are employing marketing firms to conduct Internet research to determine the brand loyalty of banking products or conducting online surveys to capture data about the customer’s experience in the branch as well as what products are missing from the bank’s current suite of products. Businesses can know how many â€Å"clicks† on their website to the feedback about the business’ service by monitoring Facebook and Twitter. 4. Why do big companies still fail in their use of information technology? What should they be doing differently? Big companies still fail in their use of information technology because they do not follow the right strategies to apply this technology to work effectively. Efficiency and effectiveness are the keys to a successful company. Companies have to be aware of the implications and abilities of f their information technology and how to apply it to their daily business  practices. They must be knowledgable and remain aware of the latest innovations in the technological machineries as well as its functions as well as its business applications. Many large companies do not realize the potential of the technology their current possess that contributes to them not seeing the value in the information technology because they are only measuring the information in terms of effeciency when they actually should be looking at effectiveness in supporting the organization’s buiness processes. Management of large companies fail to use information technology because they don’t realize how it enhances the organizational structure and culture of the company and it’s potential to increase customers and business value of the organization. Management has to play the biggest role in the change and take decisions accordingly to assist business and customer. Another important characteristic for big companies not to fail and do differently is to offer proper training for employees on the new technologies, to be able to work them in the appropriate way to deliver the right services. For example, Jackie Barretta, vice president and CIO of Con-Way, Inc. was able to benefit her cooperation from the changes she has made in adopting Agile development practices. Her actions have benefited the business as well as her employees, especially the IT department. There was less time to develop projects and a greater communication with IT as they worked jointly and determined priorities. â€Å"I made the case for change in IT by explaining how the business would benefit if we delivered the highest priority functionality faster. I also kept reiterating what was in it for them- and there was a lot† (Textbook p19) When companies’ presidents take change in consideration to benefit the company, therefore, they profited the company, the employees and the customers. 5. How can a manager demonstrate that he or she is a responsible end user of information systems? Give several examples. Managers have to be responsible end user of the information system. They have to be accountable for all aspects of corporation and take the right decisions at the right time to promote the business and its employees. Managers will have to be in charge and work thoroughly with the employees on the information system to make sure to deliver the benefits of the system. While doing so, managers have to secure the system and use ways to protect it through passwords for example to forbid the access to sensitive information. Also, if manager are working from computers not based in the company, there will be a great chance of viruses and spam that will put the information system in danger. Therefore, securing the work on the IS is a great deal before taking action or creating any change. Managers will have to monitor the work of their employees as well as the affectivity of their jobs on the system they are working on. When a manager knows of their duties and the responsibilities that are to be made for an efficient business then there will not be a problem delivering these liabilities through the information system. Being end users, managers will have to identify flaws and mistakes that take place and not over look them. When these strategies are used, it makes the job easy on both manager and employees as they work cooperatively and the security of the information system will not be likely jeopardized.